Mucopolysaccharide and protein--polysaccharide of a transplantable rat chondrosarcoma.

نویسندگان

  • H U Choi
  • K Meyer
  • R Swarm
چکیده

Two mucopolysaccharides, chondroitin 4-sulfate (97.8%) and hyaluronic acid (1.2%), were isolated after exhaustive proteolysis of a transplantable chondrosarcoma of the rat. The chondroitin 4-sulfate was fractionated into three fractions of varying degrees of sulfation and chain length. Keratan sulfate and chondroitin 6-sulfate were absent. Extraction of the fresh tumor gave two protein-polysaccharides of similar carbohydrate composition, one soluble in 0.5 M NaCl, the other insoluble. The latter was solubilized in 4 M guanidine.HCl. A dialyzable fraction from the 4 M guanidine solution may be responsible for the insolubility. Both protein-polysaccharides were antigenic and cross-reacted with similar fractions of bovine and human cartilage.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

A Mucopolysaccharide Containing Hpdroxyproline from Corn Pericarp*

A plant proteinpolysaccharide complex extracted from corn (zea mays) pericarp with 15% trichloracetic acid contains hydroxyproline as a significant constituent of its protein or polypeptide moiety. Hydroxyproline, serine, and threonine, present in nearly equimolar amounts, constitute one-third of the amino acid residues. The polysaccharide portion of this material contains glucose in major amou...

متن کامل

Production of Protein-Polysaccharide Fibers Containing Omega-3 Fatty Acids Using Electrospinning Method and Determination of Optimal Process Conditions with Response Surface Methodology

Background and Objectives: Omega-3 fatty acids are very important in the human diet for the prevention of diseases.  Materials & Methods: In the present study, the effect of whey protein concentrate (5, 6.67, 10, 13.33, 15%) and guar gum (0.1, 0.25, 0.55, 0.85, 1%) were evaluated on the qualitative properties of fish oil emulsion and electrospun fibers. Results: Increasing concentration of whey...

متن کامل

The establishment and characterization of an immortal cell line with a stable chondrocytic phenotype.

A cell line was developed from the transplantable Swarm rat chondrosarcoma (RCS) and has been maintained in continuous monolayer tissue culture for a number of years. This long term-cultured (LTC) cell line exhibits the morphological and biochemical characteristics of chondrocytes and resembles the RCS tumor by electron and light microscopy. The cell line differs from the original tumor cells i...

متن کامل

Production and Investigation of the Effects of Polysaccharide-Protein Conjugate Vaccine in Prevention of Salmonella Typhimurium Infections in Mouse Model

S.typhimurium is a gram negative bacterium causing serious enteric or extraintestinal infections in human, cattle and birds. These kinds of infections may lead to some permanent or nonpermanent complications like endophthalmitis, Guillain-Barre syndrome and osteomyelitis . Multi-resistant strains of S.typhimurium have been reported from several countries, and these infections are a major public...

متن کامل

The regenerative properties of the extracted polysaccharide from Brittle star (Ophiocoma erinaceus) on cutaneous wound in male Wistar rat

In traditional medicine, extracts of polysaccharide-containing plants are widely employed for the treatment of cutaneus wounds. The brittle star, as one of marine organisms, encompasses bioactive compounds, which confer the healing potency of damaged arms. The aim of the present study is to investigate the effects of extracted polysaccharide from the Persian Gulf brittle star )Ophiocoma erinace...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Proceedings of the National Academy of Sciences of the United States of America

دوره 68 5  شماره 

صفحات  -

تاریخ انتشار 1971